Anti-inflammatory Activity in Rats

1. Assistant Professor of Pharmacology, Bahria University Medical & Dental College, Karachi. 2. Assistant Professor of Pharmacology, Bahria University Medical & Dental College, Karachi. 3. Professor of pharmacology, United Medical & Dental College, Karachi 4. Professor of Pharmacology, Bahria University Medical & Dental College, Karachi. ABSTRACT... Objective: To evaluate anti-inflammatory activity of Salvadora persicain rats. Materials & methods: The anti-inflammatory activity of Salvadora persica was assessed in 63 albino rats of both sexes, weighing 400 gm. They were divided into three groups, A (control), B (crude drug), C (standard drug) with twenty one animals. Each group was divided into three subgroups with seven animals. Groups B &C were given crude drug and aspirin diluted in one ml of distilled water once daily orally, in doses of 300, 500, & 700 mg/kg body weight respectively, prior to induction of edema and Group A was given same volume of saline. Inflammation was induced in hind paw of rat by sub-planter injection of 0.1ml of 1% carrageenan. Paw volume was measured in terms of milliliters using plethysmometer, immediately before injection & then hourly up to 5 hours& mean was calculated. The inhibition of edema was calculated for each subgroup with respect to control group. Results: After carrageenan injection mean paw volume of control group was 5.43 ml, while that of drug treated subgroup was 4.32 ml & standard group was 4.49 ml at the dose of 700 mg/kg body weight. Comparison of crude drug with the control was found to be statistically significant only at 700 mg/kg & was comparable with the standard drug aspirin. Conclusions: Salvadora persica has shown anti-inflammatory activity by decreasing the paw volume of carageenan induced edema.